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lambda exonuclease  (New England Biolabs)


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    New England Biolabs lambda exonuclease
    Lambda Exonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+exonuclease/Lambda+Exonuclease/pmc13076092-41-11-13
    Average 98 stars, based on 1100 article reviews
    lambda exonuclease - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Incubation:

    Article Title: Molecular basis of target RNA cleavage by Cas13.
    Article Snippet: A 3 ́ adaptor was ligated with T4 RNA ligase 2, truncated KQ (NEB) according AR TI CL E IN P RE SS to manufacturers’ instructions. .. To degrade the unligated adaptors, lambda exonuclease (NEB) and 5 ́ deadenylase (NEB) were added and incubated at 30 ̊C for 30 mins followed by incubation at 37 ̊C for 30 mins. .. Reverse transcription was then performed using Maxima H minus Reverse Transcriptase (Thermo) according to manufacturers’ instructions followed by PCR to amplify the cDNA.

    other:

    Article Title: Reciprocal repulsions enforce heterotypic dendrite segregation in an olfactory circuit
    Article Snippet: Tagged UAS-caps and -ECDs were synthesized and cloned by Twist Biosciences into a 10× pUASt-attB vector.

    Generated:

    Article Title: Lamins and lineage-relevant transcription factors coordinate gene expression in lineage development
    Article Snippet: .. Single-strand DNA probes (ssDNA) were generated by treating PCR products with 7.5 μL Lambda exonuclease (NEB, M0262S 10 U/μL) in nicking enzyme buffer for 4 hours at 65°C, followed by heat inactivation at 80°C for 20 minutes. ssDNA was purified following the same ammonium acetate/ethanol precipitation as described above and was resuspended in a final volume of 40 μL molecular biology-grade water. ..

    Polymerase Chain Reaction:

    Article Title: Lamins and lineage-relevant transcription factors coordinate gene expression in lineage development
    Article Snippet: .. Single-strand DNA probes (ssDNA) were generated by treating PCR products with 7.5 μL Lambda exonuclease (NEB, M0262S 10 U/μL) in nicking enzyme buffer for 4 hours at 65°C, followed by heat inactivation at 80°C for 20 minutes. ssDNA was purified following the same ammonium acetate/ethanol precipitation as described above and was resuspended in a final volume of 40 μL molecular biology-grade water. ..

    Purification:

    Article Title: Lamins and lineage-relevant transcription factors coordinate gene expression in lineage development
    Article Snippet: .. Single-strand DNA probes (ssDNA) were generated by treating PCR products with 7.5 μL Lambda exonuclease (NEB, M0262S 10 U/μL) in nicking enzyme buffer for 4 hours at 65°C, followed by heat inactivation at 80°C for 20 minutes. ssDNA was purified following the same ammonium acetate/ethanol precipitation as described above and was resuspended in a final volume of 40 μL molecular biology-grade water. ..



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